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Material and methods Cell culture Human epithelial cancer cell lines from different head and neck regions UM-SCC-1 (floor of mouth), WSU-HN6 (tongue), and WSU-HN30 (pharyngeal) were cultured in Dulbeccos modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% antibioticantimycotic (Gibco)

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The long-term adverse health effects due to vaping are not well known, but several case reports have associated vaping with adverse respiratory, cardiovascular, neurological, and gastrointestinal health effects [13,14,15,16,17]

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