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To accomplish this, reagents and working standards were prepared according to the instructions provided by the ELISA kit (D6050, DTA00D, R&D systems, USA) and kept the room temperature for 20 min

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This is because of the sudden exposure of a persons body to smoke after several hours of fasting, some experts have warned

Smokers who littered their cigarette were on average 9.45 m away from the nearest ashtray [3] and littering evidently increased from distances of 5 m and more [5]