The cDNA was synthesised using M-MLV Reverse Transcriptase (Promega) and was 30-fold diluted with deionised water before use as a template in qRT-PCR
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However, a 2025 review noted only 3 RCTs, calling for larger trials (Nguyen et al., 2025)
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Regulatory Restrictions: As mentioned, the use of BPC-157 in horses may be subject to specific regulations depending on your location