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Instead of using 5 g of sample, extraction in 50 ml anhydrous methanol for 30 min, and titration of 10 ml of the extract, in this study we used 2 g STP to which 20 ml anhydrous methanol was added and the sample sonicated for 15 min before standing for at least 2 h to ensure complete extraction

Ben-Zaken Cohen S, Par PD, Man SF, Sin DD

Experience the pure essence of tobacco with the distinct quality of American Spirit

[DOI] [PMC free article] [PubMed] [Google Scholar] 59.Foulds J

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Additionally, taking deep inhales can increase nicotine intake, so try inhaling less deeply