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These modifications significantly extend the peptides half-life and reduce binding affinity to IGF-binding proteins compared to native IGF-1

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The chemiluminescent readout was obtained using SuperSignal West Femto maximum sensitive substrate (Thermo Scientific GmbH, Schwerte, Germany) with a c400 Azure imaging system (lowest sensitivity, 10 s exposure time)

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doi: 10.1016/j.jmb.2024.168888 61 WangF.ZhangW.YinL.HuangC.ZhaoH