In this study, the authors demonstrated that siRNA-mediated knockdown of HNF4 initiates a microRNA-inflammatory feedback loop that continuously suppresses HNF4 expression and sustains a stable phenotype of tumorigenesis 56
RNA extraction and RT-PCR for gene expression analysis To determine the effect of HT stress on the transcription levels of Calvin cycle enzyme genes and antioxidant enzyme genes and their changes after GSH application, we tested photosynthetic genes including those encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase ( RCA ), RBCL and RBCS involved in CO 2 fixation
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