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Furthermore, a two-way ANOVA results revealed a nonsignificant change in GABA levels in the midbrain, thalamus and hypothalamus, and hippocampus between the duration of IF and the diet regimen intervention ( F = 2.873, p = 0.076), ( F = 2.216, p = 0.131), and ( F = 0.848, p = 0.441), respectively