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Characteristics of Users of Cigars, Bidis, and Kreteks and the Relationship to Cigarette Use
The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h
Whereas adult smoking has decreased in the last four decades, teen smoking, while first decreasing after the mid-seventies, has risen dramatically since 1990
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