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Results Dose dependent reduction of Drosophila locomotor activity by e-nicotine To quantify the sensitivity of adult female flies to e-nicotine and to determine the appropriate concentration of e-nicotine to be further used in our experiments, the locomotor behavior of female flies following their exposure to different concentrations of e-nicotine (1, 5 or 10 mM) was measured using a negative geotaxis assay 36

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Tobacco barns for housing the crop were in use by the 1620s