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Journal of Alternative and Complementary Medicine (New York, N.Y.), 21 (1), 2230

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Over time, the duration and frequency of these flares may increase, resulting in chronic gouty arthritis and subcutaneous deposits of crystals, referred to as tophi, both of which can lead to the destruction of joints and musculoskeletal disability

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Hansen et al [17] used Hanks Balanced Salt Solution (HBSS) containing 5% FCS and 2 mM EDTA for both, while Cervantes-Barragan et al [15] replaced the HBSS with PBS and Luckashenak et al [16] used PBS for suspending the cells and HBSS for diluting the OptiPrep (both containing 0.2% BSA and 2 mM EDTA