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TUNEL assay for apoptosis detection Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using VasoTACS In Situ Apoptosis Detection Kit (482630-K, Trevigen, Gaithersburg, MD) following the manufacturers instructions

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The American Legacy Foundation surveyed a national sample of 1000 smokers and found that most (74.1 %) admitted disposing of butts on the ground or out of a car window at least once in their lives [9]

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