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MesoScale analysis Undiluted plasma samples were analyzed using a Mesoscale Discovery (MSD) V-PLEX Pro-inflammatory Panel 1 (human) kit for IL-6 quantification and Cytokine Panel 1 (human) kits for IL-17 and VEGF quantification (K15049D & K15050D, Mesoscale Discovery, Gaithersburg, MD) according to the manufacturers instructions and as previously described 20,76

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Laboratories regulate such variables (humidity, temperature, puff interval) so they are comparable