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As noted above, this is expected and aligns with the protocols design

Goncalves, N

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This is particularly crucial for kidney infections, where prompt intervention can prevent complications such as kidney damage or sepsis

Crossing strategies are: (1) bypassing intranasal route, (2) lipid modifications (Dihexa), (3) active transporters (insulin, transferrin), (4) vehicles like nanoparticles or exosomes

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min