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For Hoechst 33342/PI staining, HAECs (10 5 cells/well) were cultured in a 12-well plate and were treated with test drugs or siRNA for the duration as to be specified in the appropriate section

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30,31 Because of changes that were described previously to the questions for the use of hallucinogens, inhalants, and methamphetamine and the misuse of prescription drugs, the 2016 estimates for overall SUD (i.e., for alcohol or illicit drugs), any illicit drug use disorder, and SUDs for these specific illicit drugs are not comparable with the estimates from years prior to 2015

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