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Coomassie staining showed a single protein band with the expected 88.5 kDa size (Figure 1B)

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The ROS produced are converted to peroxynitrite, which has a very strong cytotoxic effect when combined with NO, resulting in direct damage to vascular wall cells and a decrease in the biological activity of NO in vascular endothelial cells and vascular smooth muscle cells [68]

P.PerretJ.-L.LeroyP.MazurovA.PeitschM