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List 1: Histopathological features of epithelial dysplasia 1) Abnormal variation in nuclear size (anisonucleosis) and shape (nuclear pleomorphism) 2) Change in cell size (anisocytosis) and shape (cellular pleomorphism) 3) Loss of polarity of the basal cells 4) Presence of more than one layer of cells having a basaloid appearance 5) Increased nuclearcytoplasmic ratio 6) Drop-shaped rete processes 7) Irregular epithelial stratification 8) Increased number of mitotic figures (a few abnormal mitoses may be present) 9) Presence of mitotic figures in the superficial half of the epithelium 10) Nuclear hyperchromatism 11) Enlarged nucleoli 12) Reduction of cellular cohesion 13) Keratinization of single cells or cell groups in the prickle layer These histopathological changes are clinically used as a confirmatory test for leukoplakia positivity and for dysplastic changes (Kramer et al., 1978

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When working with complex tissue such as whole-brain or blood samples, it is essential to correct for cellular heterogeneity