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In answer to the more direct question of how much nicotine per day is safe, a reasonable starting point might be, "How much nicotine might it take to poison someone?" The ECHA currently gives an "acute toxicity estimate" (ATE) for nicotine via the oral route to be 5 mg/kg bw [7] , meaning 5mg of nicotine for every kilogram of bodyweight of the person consuming the nicotine

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TRIM47 targets PPM1A for ubiquitination, leading to its degradation via the 26S proteasome complex, which in turn increases the phosphorylation of Smad2/3 and contributes to lung fibrosis (110)