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Subsequently, the sorted CD4 + and CD8 + T cells were activated by TransAct microspheres presenting anti-CD3/CD28 antibodies (Miltenyi Biotec) and cultured in Prime-XV T-cell medium (Irvine Scientific, Santa Ana, CA, USA) with 400 IU/mL interleukin (IL)-2 (Shandong Quangang Co., Shandong, China)

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Liu et al., 2020

doi: 10.3389/fimmu.2021.720109 146 KobayashiE