Formamide BAC Unit Definition: One unit of

Unit Definition: One unit of RNase R converts 1 µg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C under standard assay conditions

RNase I does not have a base preference and digests between all dinucleotide pairs in single-stranded RNA

Unit Definition: One unit of RNA 5´ Polyphosphatase releases 1 nmol of inorganic phosphate from ATP in 1 hour at 37°C under standard assay conditions

use a freshly prepared lysozyme solution (10 mg/ml) in 10 mM Tris-HCl

Quality Control: T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free of detectable exo- and endonuclease and RNase activities

Formamide BAC Unit Definition: One unit of