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The enzyme is supplied with a 10X Reaction Buffer
MC1061 F- Electrocompetent Cells
and BAC DNA purifications
Unit Definition: One unit of CircLigase II enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase II Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
Copy-number of clones is under tight control of an inducible promoter linked to the trfA gene
EZ-10 Spin Column Plasmid DNA Mini-preps Kit, Low Copy Plasmid - 50 preps size:50 preps The enzyme is supplied withThe EZ 10 Spin Column Kits provide a simple and efficient method for purification of plasmid DNA, extraction of DNA from agarose gels, and purification of DNA from enzymatic reactions such as PCR or restriction enzyme digestions. The DNA is selectively adsorbed in silica gel based EZ 10 column and other components are washed away. The DNA is then eluted off the column and can be used for any downstream applications. The purification method used in