DNA Fingerprinting size:For 6 groups of 4-5 or 24-30 students. bacterial colonies can be grown

bacterial colonies can be grown on solid agar plates to show the vast numbers of bacteria present in the mouth

A processive 5´ to 3´ riboexonuclease that specifically digests RNA with 5´-monophosphate ends

CopyCutter EPI400 Chemically Competent E

The following laboratory activity is adapted from “Laboratory 4h: DNA Extraction from Bacteria” from Biotechnology: Laboratory Manual by Ellyn Daugherty

0 A260 (~60 µg) of sonicated salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50 mM Tris-HCl (pH 8

DNA Fingerprinting size:For 6 groups of 4-5 or 24-30 students. bacterial colonies can be grownTodays media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo crime scene. Students will use specialized molecules to cut the DNA into fragments, which can then be visualized by a process known as electrophoresis. The resulting