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cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier
mixture of 3 µg of digested bacterial chromosomal DNA and 500 ng of uncut plasmid before Plasmid-Safe DNase treatment
Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4
10 mM EDTA
and the same high promoter specificity as wild-type T7 RNA Polymerase
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects cloni 10G SUPREME Electrocompetent CellsTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES