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Reverse transcription was done using the RevertAid first-strand cDNA synthesis Kit (Thermo Fisher Scientific, USA) and quantitative PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) on a Bio-Rad CFX96-Real-Time PCR Detection System

However, iron chelators are relatively small molecules compared with proteins, and no significant neurotoxicity or immunogenicity due to IN DFO has been reported

Starting from the first day of tumor formation, the length (L) and width (W) of the tumor were measured with vernier calipers every 2 days to calculate tumor size

M., Hirst, W

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