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The protocol is extremely flexible and easily adjusted if there are varying volumes to be purified
prior their being quality tested for optimal and reproducible impedance measurements
EconoTaq PLUS 2X Master Mix offers the same great performance without the dyes
biocompatibility and sterility
RNAs with a 5´-diphosphorylated end are also converted to 5´-monophosphorylated RNA by RNA 5´ Polyphosphatase:
CircLigase-ssDNA Ligase, 1,000 U Chemicals The protocol is extremely flexibleThermostable ligase that catalyzes the intramolecular ligation (i. e. circularization) of ssDNA templates Intramolecular ligation of single stranded DNA ends containing 5' phosphates and 3' hydroxyl groups ATP dependent, catalytic enzyme Circularizes ssDNA >30 bases long Compatible with high temperature ligation reactions due to thermostability of CircLigase enzyme Applications Production of ssDNA templates for rolling circle replication or rolling
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