OverExpress-C43(DE3) Chemically Competent Cells (SOLOs) ECloni5alpha Crude extracts of plasmid DNA

Crude extracts of plasmid DNA were prepared from cells grown in selective media and analyzed by agarose gel electrophoresis

The reduced number of protocol steps makes it extremely easy-to-use where a simple yet efficient isolation method is required

Flexible: Use newly phosphorylated nucleic acids in a variety of applications such as cloning

Easy to handle and quick to use

0 × 108 145-kb BAC Clone Not tested 7 × 107 13

OverExpress-C43(DE3) Chemically Competent Cells (SOLOs) ECloni5alpha Crude extracts of plasmid DNAOverExpress C41(DE3) and C43(DE3) Competent Cells Express genes cloned into any T7 vector with these BL21(DE3) derivatives Effective in expressing toxic & membrane proteins Recommended for membrane proteins with T7 vector E. coli BL21(DE3) strains, like Lucigens E. cloni EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e. g., pET or Lucigens pSMART cDNA vectors). However, in some cases expression is