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Unit Definition: One unit of MasterAmp Tth DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions
500 ng of uncut plasmid DNA
Tips for use:
An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening
CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence
E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells BAC-Optimized Replicator Unit Definition: One unit ofE. cloni EXPRESS BL21(DE3) Competent Cells Best value for routine protein expression available as Electrocompetent and Chemically Competent Cells. Lucigens E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25 1,000 fold relative to competitors preparations of BL21 cells (Figure 1), which is essential for construction of
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