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The burning of tobacco produces ROS in the gas phase inhaled by smokers, as part of the mainstream smoke (57)

The non-enzymatic antioxidants are the scavengers of ROS and RNS [59]

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Subsequently, 100 L of coelenterazine (10 M in PBS pH 7.4) was added, and the luciferase activity was determined using a luminescence microplate reader (Tecan Infinite M-Plex