Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) ChIP-seq and FFPE and cell

ChIP-seq and FFPE and cell free DNA samples

The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3)

subcloning

PCR amplification of DNA and one-step RT-PCR of RNA

Avoid T1 and T5 phage contamination with tonA mutation

Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) ChIP-seq and FFPE and cellDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease