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In a cervical cancer cell line (HeLa), the compound reduced cell proliferation in a concentration- and time-dependent way and lowered expression of phospho-Akt and SIRT1, two proteins tied to tumor progression, while a non-cancer reference cell line was less affected

Multiple compounds targeting the same pathways may cause excessive stimulation or unpredictable interactions

AOD 9604 is meant for subcutaneous injection, not intramuscular

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This inherent biocompatibility means the body recognizes it and knows how to use it, minimizing the risk of adverse reactions often associated with foreign substances

Topical protocols deliver the peptide directly to dermal tissue at the application site, achieving high local concentrations without systemic distribution