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Coffee enemas have almost no scientific backing and have been linked to problems with electrolyte balance, intestinal damage, inflammation, and infection

536 Prenyldiphosphate synthase subunit 2 (PDSS2) may inhibit the formation of atherosclerotic plaque by activating Nrf2 and reducing ferroptosis in human coronary artery endothelial cells

Hong SW, Lee HJ, Han K, et al

This approach helps maintain DSIP's stability and efficacy for up to four weeks when stored in refrigeration between 36 to 46F (2 to 8C)

The ranges in this guide lean on that preclinical literature plus what providers observe in practice