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Eur J Med Chem (2022) 236:114324

After blocking with 20% non-fat milk at room temperature for 1 h, the membranes were incubated with primary antibodies against GPX4 (1:1,000), Nrf2 (1:1,000), HO-1 (1:1,000), HMGB1 (1:1,000) and GAPDH (1:1,000) overnight at 4C

This is because the FDA regulates the testing and approval of drugs, but not how doctors use drugs to treat their patients