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See a gastroenterologist if symptoms persist or are severe A visit to a specialist is warranted if bloating is followed by symptoms like: Sharp pain Fever Bloody stools Unexplained weight loss Severe nausea and vomiting Healthy dietary practices and over-the-counter medication may work for mild pain and bloating, but it is dangerous to dismiss severe symptoms

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Subsequently, the cells were incubated with anti-caspase-1 antibody at 4 C overnight, followed by incubation with Alexa Fluor-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) in the dark for 1 h

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