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Membranes were incubated overnight at 4 C with Cleaved Caspase-3 (Asp175) (Cell Signalling Technology) and with peroxidase-linked anti-rabbit IgG or anti-mouse IgG secondary antibodies (GE Healthcare Life science) for 1 h at room temperature

Both investigations documented a substantial rise in malnourished patients after therapy using a serum albumin level of less than 35 g/L and a body mass index of less than 18.5 kg/m 2 as markers

A P value of 0.05 or less was considered statistically significant

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Why Site Rotation Matters For long-term injection success, site rotation is critical