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For in vivo research, prepare fresh working solutions at the time of administration and maintain on ice

The tightness of the BBB precludes the entrance of most pharmaceuticals into the brain via passive transcytosis, except for hydrophilic compounds which have a mass lower than 150 Da and highly hydrophobic compounds which have a mass lower than 400600 Da

Active transport of sodium as the source of electric current in the short-circuited isolated frog skin

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Research is also needed to meet the challenges of identifying the active compounds in the plants, and there should be research-based evidence on the safety and efficacy of both whole herbs and extracted compounds (Wachtel-Galor and Benzie, 2011)