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While genotypes were not investigated, blood markers for antioxidant enzymes were measured at baseline and then acutely for up to three hours
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For IRE1 cleavage assay, purified RNA substrates were first incubated with inhibitor or vehicle (DMSO) for 30 min on ice, then incubated with recombinant GST-tagged human IRE1 (aa 465-977 corresponding to the kinase/ribonuclease domain, 11905-H20B, Sino Biological) in reaction buffer (40 mM HEPES pH 7.0, 10 mM Mg(OAc) 2 , 50 mM KOAc, 5 mM DTT, 1 M ADP) for 30 minutes at 30 C
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For instance, polymorphisms in inflammatory genes, such as interleukin-1 -31T/C, interact synergistically with tobacco exposure and alcohol intake to elevate HCC risk [44]
1, May 2019, p
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