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de L., Lima, R

RNA Isolation Cells were washed with PBS without Ca 2+ and Mg 2+ (Sigma) and each sample (1 10 6 cells) was lyzed with 1 ml QIAzol (QIAGEN)

Acoustic calibration was performed with TDT software (SigCal) and thresholds were expressed as dB SPL in conditions identical to that of threshold recordings in animals

In clinical studies the numbers are unusual for a sleep aid: DSIP increased total sleep time by about 59% in one trial, normalized sleep in people with chronic insomnia in another, and, most strikingly, several of those patients kept normal sleep for 3 to 7 months after their last injection

Pediatr Nephrol 30:775781 Cheng R, Cooper A, Kench J, Watson G, Bye W, McNeil C et al (2015) Ipilimumab-induced toxicities and the gastroenterologist