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Wipe the vial stopper with a fresh alcohol pad

The crystallization that occurs during freezing permanently damages the peptide structure in ways that gentle thawing cannot reverse

01 Bring the vial to room temperature Take the vial out of the freezer and let it sit on the counter for 15 to 20 minutes

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doi: 10.4049/jimmunol.150.11.4733 44 WoltersPJRaymondWWBlountJLCaugheyGH