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ROS production was identified in chloroplasts and believed to be generated from photosystem I and II activity which supports the findings of Foyer (2018)

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doi: 10.1146/annurev-nutr-082018-124337

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A wound chamber creates a walled-off, initially cell-free space with a defined volume

Third, the causal relationship between ferroptosis and stone pathogenesis lacks rigorous validationexisting data only confirm oxalate induces ferroptosis, but it remains unclear whether ferroptosis directly promotes crystal adhesion, tubular injury, or inflammatory responses (key steps in stone formation) or is merely a secondary consequence of oxalate toxicity