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Cytokines, graves' disease, and thyroid-associated ophthalmopathy

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For LCMS analysis, metabolite extracts were analysed by high-resolution mass spectrometry with an Orbitrap Exploris 240 (Thermo) coupled to a Vanquish Flex liquid chromatography system (Thermo)

To each cell pellet, 200 L of the extraction solution and 10 L of the internal standard (IS) mixture were added

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