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This inhibition can lead to: Enhanced Cellular Metabolism: By inhibiting NNMT, 5-Amino-1MQ can increase levels of NAD+, a coenzyme crucial for energy metabolism and cellular function [4]

Briefly, whole-cell lysate was obtained by cell lysis buffer in the presence of a protease inhibitor cocktail (Sigma-Aldrich)

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For comparative lipid penetration studies, both are reference compounds

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